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KMID : 0855520100230040199
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Korean Journal of Physical Anthropology
2010 Volume.23 No. 4 p.199 ~ p.206
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Microscopic Observation of Decomposition-inhibition Effect in GSE (Grapefruit Seed Extract) in Rat Liver
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Hwang Kyu-Sung
Lim Do-Seon
Choi Ki-Ju
Seo Youn-Kyoung
Paik Doo-Jin
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Abstract
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Regarding to preserve the cadaver, formaldehyde has been used as a major preservative. However, the usage of formaldehyde has been considered by its harmful effects such as the disturbing ordor, toxicities and limitations to use. Therefore we studied the effect of decomposition-inhibition which is a natural product, grapefruit seed extract (GSE).
Concerning the preservative activity, we sacrificed 8 week old male SD rat and collected liver. Using liver tissues, we treated GSE as a time dependant manner under 37℃, 80±5% humidity conditions. To confirm GSE effects, we applied light and electron microscopic analysis.
In results, we observed GSE attenuated the morphological changes and putrefaction of liver tissues more than 3 days. Herein, we introduced the potential substitute of formaldehyde to preserve the cadaver as well as animal tissues.
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KEYWORD
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GSE (Grapefruit Seed Extract), Preservative, Decomposition-inhibition, Rat liver, Microscopic analysis
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